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Singh, Poonam
- Micromass Culture: a Recent in vitro System for Testing Embryotoxic Potential of Chemicals
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Authors
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1 Division of Toxicology, Central Drug Research Institute, Lucknow- 226001, IN
1 Division of Toxicology, Central Drug Research Institute, Lucknow- 226001, IN
Source
Indian Journal of Science and Technology, Vol 2, No 8 (2009), Pagination: 53-56Abstract
Drugs and other chemicals must be evaluated for their teratatological potential before releasing them in the market. One of the model test system that is used in screening of embryotoxic chemicals in vitro, is the micromass culture test. The test involves exposing the rat embryo limb cells or cells of central nervous system (CNS) to various test agents for different time intervals and estimating the inhibition of cell differentiation in these cell cultures. The technique can be best used for identifying strong embryotoxic chemicals due to its sensitivity to a number of classes of potent teratogens and insensitivity to non-teratogens.Keywords
Embryotoxic, in vitro, Micromass, Teratogen, TeratogenicityFull Text
References
- Best JB and Morita M (1982) Planarions as a model system for in vitro teratogenesis studies. Teratogenesis Carcinogen Mutagen. 2, 277-291.
- Braun AG, Nichinson BB and Horowiez PB (1982) Inhibition of tumour cell attachment to concanavalin A-coated surfaces as an assay for teratogenic agents: approaches to validation. Teratogenesis Carcinogenesis Mutagenesis. 2, 343-354.
- Brown NA and Wiger R (1992) Comparison of rat and chick limb bud micromass cultures for developmental toxicity screening. Toxicol In Vitro. 6, 101-108.
- Brown LP, Flint OP, Orton TC and Gibson GG (1986) In vitro metabolism of teratogens by differentiating rat embryo cells. Food Chem. Toxicol. 24, 737-782.
- Bournias-Vardiabasis N and Teplitz RL (1982) Use of Drosophilla embryo cell cultures as an in vitro teratogen assay. Teratogenesis Carcinogen Mutagen. 2, 335-341.
- Flint OP (1983) A micromass culture method for rat embryonic neural cells. J. Cell. Sci. 61, 247-262.
- Flint OP, Orton TC and Ferguson RA (1984) Differentiation of rat embryo cells in culture: Response following acute maternal exposure to teratogens and non-teratogens. J. Appl. Toxicol. 4, 109-116.
- Flint OP (1993) In vitro tests for teratogens: Desirable endpoints, test batteries and current status of the micromass teratogen test. Repr. Toxicol. 7, 103-111.
- Flint OP and Boyle FT (1986) Structureteratogenecity relationships among antifungal triazoles. In: Ryley JF, ed. Handbook of experimental pharmacology. Volume 96: chemotherapy of fungal disease. Berlin: Springer-Verlag, pp: 231-249.
- Guiney PD, Lech, JJ and Peterson RE (1980) Distribution and elimination of polychlorinated orpphenyl during early life stages of rainbow trout. Xenobiotica. 7, 633-640.
- Jorg GK Handschel, Rita A Depprich, Norbert R Kubler, Hans-Peter Wiesmann, Michel and Ulrich Meyer (2007) Prospects of micromass culture echnology in tissue engineering. Head & Face Medicine. 3, 4.
- Kistler A, Tsuchiya T, Tsuchiya M and Klaus M (1990) Teratogenicity of arotinoids (retinoids) in vivo and in vitro. Archiv. Toxicol. 64, 616-622.
- Koelman HJS, Jongeling AJ, van Erp YHM, Weterrings PJM, Koopmans ME, Joosten HF, van der Dobbelsteen DJ, van der Aa, EM and Yih, TD (1991) International ring validation of the in vitro micromass teratogenicity test: preliminary results of 3 Dutch laboratories. Teratology . 44, 30A.
- Minta M (2003) Identification of teratogens in cell culture. II. Laboratory values of rat embryo midbrain studied by micromass test with penicillin G and 5- fluorouracil. Bull. Vet. Inst. Pulawy. 47, 533-540.
- Minta M and Wlodarczyk B (2003) Effects of some environmental contaminants on differentiation in micromass culture of rat embryo limb bud cells. Repr. Toxicol. 17(4), 500.
- Minta M., Wilk I and Zmudzki, J (2004) Embryotoxicity of carbendazim in rat and hamster micromass cultures. Bull. Vet. Inst. Pulawy. 48, 481-484.
- Mummery CL, van der Brink CE, van der Saag PT and De Laal SW (1984) A short term screening test for teratogens using differentiating neuroblastoma cells. In vitro Teratology. 29, 271-279.
- New DAT (1978) Whole embryo culture and the study of mammalian embryos during embryogenesis. Biol. Rev. 53, 81-122.
- Prall R.M, Grove RI and Wilis WD (1982) Prescreening for environmental teratogens using cultured mesenchymal cells from the human embryonic palate. Teratogenesis Carcinogenesis Mutagenesis. 2, 313-318.
- Johnson EM and Gabel BEG. (1983) An artificial embryo for detection of abnormal developmental biology. Fund. App. Toxicol .3, 243-249.
- Tsuchiya T, Takahashi A, Asada S, Takakubo F, Ohsumi-Yamashita N and Eto, K (1991) Comparative study of embryotoxic action of ethylenethiourea in rat whole embryo and embryonic cell culture. Teratology. 43, 319-324.
- Tsuchiya T, Nakamura A, Iio T and Takahashi A (1991) Species differences between rats and mice in the teratogenic action of ethylenethiurea: in vivo in vitro tests and teratogenic activity of sera using an embryonic cell differentiation system. Toxicol. Appl. Pharmacol. 109, 1-6.
- Uphill PF, Wilkins SR and Allen JA (1990) In vitro micromass teratogen test: Results from a blind trial of 25 compounds. Toxicol. In Vitro. 4 (4-5).
- Waltan BT (1983) Use of the cricket embryo (Acheta domesticus) as an invertebrate teratology model. Fund. App. Toxicol . 3, 233-236.
- Ward SJ and Newall DR (1990) Micromass test: is it subject to strain variation? Toxicol. In Vitro 4, 620-622.
- Wittaker SG and Faustman EM (1991) Effects of albendazole and albendazole sulfoxide on cultures of differentiating rodent embryonic cells. Toxicol. Appl. Pharmacol. 109, 73-84.
- Umansky R (1966) The effect of cell population density on the developmental fate of reaggregating mouse limb bud mesenchyme. Dev. Biol. 13, 31-56.
- Zurich MG, Honegger P, Schilter B, Costa LG and Monnet-Tschudi F (2000) Use of aggregating brain cell cultures to study developmental effects of organophosphorous insecticides. Neurotoxicology .21(4), 599-605.